Molecular biology

Why a unique guide still has off-targets

Name: Why a unique guide still has off-targets
Uploaded: 2026-08-20T12:11:58.425Z
Description: There are 4²⁰ possible 20-mers and only three billion bases, so a guide must be unique. It is — until you allow three mismatches, and the target becomes thirty thousand sequences instead of one.

There are 4²⁰ possible 20-mers and only three billion bases, so a guide must be unique. It is — until you allow three mismatches, and the target becomes thirty thousand sequences instead of one.

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